polyclonal pc goat anti c3 antibody Search Results


90
Cappel Laboratories peroxidase-labeled anti-human c3 antibody
Peroxidase Labeled Anti Human C3 Antibody, supplied by Cappel Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cosmo Bio USA polyclonal hrp-conjugated goat anti-mouse c3 antibody gc3-90p-z
Polyclonal Hrp Conjugated Goat Anti Mouse C3 Antibody Gc3 90p Z, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cappel Laboratories polyclonal goat anti–human c3-fitc antibody
Detection of C3 fragments in mouse and human blood following incubation ex vivo at 37°C. (A) The level of C3 fragments in mouse blood collected directly into heparin and EDTA (T 0 ) or collected into heparin and incubated at 37°C for 10 min before addition of EDTA (T 10 ). The histograms represent the amount of C3 fragments detected on B220 + B cells following incubation of splenic lymphocytes with T 0 plasma (filled histogram) or T 10 plasma (open histogram), as measured by immunofluorescence flow cytometry. (B) Western blot analysis of C3 fragments in mouse plasma, as collected in A. C3 was immunoprecipitated from plasma samples with <t>polyclonal</t> anti–mouse C3 antibody conjugated Sepharose beads, and the Western blot was probed with biotin-labeled polyclonal anti–mouse C3 Ab and developed using streptavidin-HRP. Position of intact C3α and C3β chains and the C3dg fragments, that bind to CR1/2 on B cells (A), is indicated. (C) The level of C3 fragments in human blood collected directly into heparin and EDTA (T 0 ) or into heparin and incubated at 37°C for 30 min before addition of EDTA (T 30 ). The histograms represent the amount of C3 (solid line) or C3d (broken line) fragments detected on Raji cells after incubation with T 0 plasma (filled histogram) or T 30 plasma (open histograms), as measured by immunofluorescence flow cytometry. (D) The rate of formation of C3 fragments in blood from C57BL/6 mice collected into either heparin (□) or hirudin (▪) is depicted, as measured by the binding of C3 fragments to B220 + spleen cells using immunofluorescence flow cytometry, as shown in A. Error bars represent ± SD of means of triplicate assays.
Polyclonal Goat Anti–Human C3 Fitc Antibody, supplied by Cappel Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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GeneTex goat anti-mouse c3 polyclonal antibody gt x77263
Detection of C3 fragments in mouse and human blood following incubation ex vivo at 37°C. (A) The level of C3 fragments in mouse blood collected directly into heparin and EDTA (T 0 ) or collected into heparin and incubated at 37°C for 10 min before addition of EDTA (T 10 ). The histograms represent the amount of C3 fragments detected on B220 + B cells following incubation of splenic lymphocytes with T 0 plasma (filled histogram) or T 10 plasma (open histogram), as measured by immunofluorescence flow cytometry. (B) Western blot analysis of C3 fragments in mouse plasma, as collected in A. C3 was immunoprecipitated from plasma samples with <t>polyclonal</t> anti–mouse C3 antibody conjugated Sepharose beads, and the Western blot was probed with biotin-labeled polyclonal anti–mouse C3 Ab and developed using streptavidin-HRP. Position of intact C3α and C3β chains and the C3dg fragments, that bind to CR1/2 on B cells (A), is indicated. (C) The level of C3 fragments in human blood collected directly into heparin and EDTA (T 0 ) or into heparin and incubated at 37°C for 30 min before addition of EDTA (T 30 ). The histograms represent the amount of C3 (solid line) or C3d (broken line) fragments detected on Raji cells after incubation with T 0 plasma (filled histogram) or T 30 plasma (open histograms), as measured by immunofluorescence flow cytometry. (D) The rate of formation of C3 fragments in blood from C57BL/6 mice collected into either heparin (□) or hirudin (▪) is depicted, as measured by the binding of C3 fragments to B220 + spleen cells using immunofluorescence flow cytometry, as shown in A. Error bars represent ± SD of means of triplicate assays.
Goat Anti Mouse C3 Polyclonal Antibody Gt X77263, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck & Co polyclonal goat c3 antiserum
Detection of C3 fragments in mouse and human blood following incubation ex vivo at 37°C. (A) The level of C3 fragments in mouse blood collected directly into heparin and EDTA (T 0 ) or collected into heparin and incubated at 37°C for 10 min before addition of EDTA (T 10 ). The histograms represent the amount of C3 fragments detected on B220 + B cells following incubation of splenic lymphocytes with T 0 plasma (filled histogram) or T 10 plasma (open histogram), as measured by immunofluorescence flow cytometry. (B) Western blot analysis of C3 fragments in mouse plasma, as collected in A. C3 was immunoprecipitated from plasma samples with <t>polyclonal</t> anti–mouse C3 antibody conjugated Sepharose beads, and the Western blot was probed with biotin-labeled polyclonal anti–mouse C3 Ab and developed using streptavidin-HRP. Position of intact C3α and C3β chains and the C3dg fragments, that bind to CR1/2 on B cells (A), is indicated. (C) The level of C3 fragments in human blood collected directly into heparin and EDTA (T 0 ) or into heparin and incubated at 37°C for 30 min before addition of EDTA (T 30 ). The histograms represent the amount of C3 (solid line) or C3d (broken line) fragments detected on Raji cells after incubation with T 0 plasma (filled histogram) or T 30 plasma (open histograms), as measured by immunofluorescence flow cytometry. (D) The rate of formation of C3 fragments in blood from C57BL/6 mice collected into either heparin (□) or hirudin (▪) is depicted, as measured by the binding of C3 fragments to B220 + spleen cells using immunofluorescence flow cytometry, as shown in A. Error bars represent ± SD of means of triplicate assays.
Polyclonal Goat C3 Antiserum, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Valiant Co Ltd complement c3 polyclonal antibody
Detection of C3 fragments in mouse and human blood following incubation ex vivo at 37°C. (A) The level of C3 fragments in mouse blood collected directly into heparin and EDTA (T 0 ) or collected into heparin and incubated at 37°C for 10 min before addition of EDTA (T 10 ). The histograms represent the amount of C3 fragments detected on B220 + B cells following incubation of splenic lymphocytes with T 0 plasma (filled histogram) or T 10 plasma (open histogram), as measured by immunofluorescence flow cytometry. (B) Western blot analysis of C3 fragments in mouse plasma, as collected in A. C3 was immunoprecipitated from plasma samples with <t>polyclonal</t> anti–mouse C3 antibody conjugated Sepharose beads, and the Western blot was probed with biotin-labeled polyclonal anti–mouse C3 Ab and developed using streptavidin-HRP. Position of intact C3α and C3β chains and the C3dg fragments, that bind to CR1/2 on B cells (A), is indicated. (C) The level of C3 fragments in human blood collected directly into heparin and EDTA (T 0 ) or into heparin and incubated at 37°C for 30 min before addition of EDTA (T 30 ). The histograms represent the amount of C3 (solid line) or C3d (broken line) fragments detected on Raji cells after incubation with T 0 plasma (filled histogram) or T 30 plasma (open histograms), as measured by immunofluorescence flow cytometry. (D) The rate of formation of C3 fragments in blood from C57BL/6 mice collected into either heparin (□) or hirudin (▪) is depicted, as measured by the binding of C3 fragments to B220 + spleen cells using immunofluorescence flow cytometry, as shown in A. Error bars represent ± SD of means of triplicate assays.
Complement C3 Polyclonal Antibody, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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complement c3 polyclonal antibody - by Bioz Stars, 2026-02
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90
Cappel Laboratories polyclonal antibody fluorescein conjugated goat f(ab')2 anti-mouse c3
Detection of C3 fragments in mouse and human blood following incubation ex vivo at 37°C. (A) The level of C3 fragments in mouse blood collected directly into heparin and EDTA (T 0 ) or collected into heparin and incubated at 37°C for 10 min before addition of EDTA (T 10 ). The histograms represent the amount of C3 fragments detected on B220 + B cells following incubation of splenic lymphocytes with T 0 plasma (filled histogram) or T 10 plasma (open histogram), as measured by immunofluorescence flow cytometry. (B) Western blot analysis of C3 fragments in mouse plasma, as collected in A. C3 was immunoprecipitated from plasma samples with <t>polyclonal</t> anti–mouse C3 antibody conjugated Sepharose beads, and the Western blot was probed with biotin-labeled polyclonal anti–mouse C3 Ab and developed using streptavidin-HRP. Position of intact C3α and C3β chains and the C3dg fragments, that bind to CR1/2 on B cells (A), is indicated. (C) The level of C3 fragments in human blood collected directly into heparin and EDTA (T 0 ) or into heparin and incubated at 37°C for 30 min before addition of EDTA (T 30 ). The histograms represent the amount of C3 (solid line) or C3d (broken line) fragments detected on Raji cells after incubation with T 0 plasma (filled histogram) or T 30 plasma (open histograms), as measured by immunofluorescence flow cytometry. (D) The rate of formation of C3 fragments in blood from C57BL/6 mice collected into either heparin (□) or hirudin (▪) is depicted, as measured by the binding of C3 fragments to B220 + spleen cells using immunofluorescence flow cytometry, as shown in A. Error bars represent ± SD of means of triplicate assays.
Polyclonal Antibody Fluorescein Conjugated Goat F(Ab')2 Anti Mouse C3, supplied by Cappel Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Detection of C3 fragments in mouse and human blood following incubation ex vivo at 37°C. (A) The level of C3 fragments in mouse blood collected directly into heparin and EDTA (T 0 ) or collected into heparin and incubated at 37°C for 10 min before addition of EDTA (T 10 ). The histograms represent the amount of C3 fragments detected on B220 + B cells following incubation of splenic lymphocytes with T 0 plasma (filled histogram) or T 10 plasma (open histogram), as measured by immunofluorescence flow cytometry. (B) Western blot analysis of C3 fragments in mouse plasma, as collected in A. C3 was immunoprecipitated from plasma samples with polyclonal anti–mouse C3 antibody conjugated Sepharose beads, and the Western blot was probed with biotin-labeled polyclonal anti–mouse C3 Ab and developed using streptavidin-HRP. Position of intact C3α and C3β chains and the C3dg fragments, that bind to CR1/2 on B cells (A), is indicated. (C) The level of C3 fragments in human blood collected directly into heparin and EDTA (T 0 ) or into heparin and incubated at 37°C for 30 min before addition of EDTA (T 30 ). The histograms represent the amount of C3 (solid line) or C3d (broken line) fragments detected on Raji cells after incubation with T 0 plasma (filled histogram) or T 30 plasma (open histograms), as measured by immunofluorescence flow cytometry. (D) The rate of formation of C3 fragments in blood from C57BL/6 mice collected into either heparin (□) or hirudin (▪) is depicted, as measured by the binding of C3 fragments to B220 + spleen cells using immunofluorescence flow cytometry, as shown in A. Error bars represent ± SD of means of triplicate assays.

Journal: The Journal of Experimental Medicine

Article Title: Continual Low-Level Activation of the Classical Complement Pathway

doi:

Figure Lengend Snippet: Detection of C3 fragments in mouse and human blood following incubation ex vivo at 37°C. (A) The level of C3 fragments in mouse blood collected directly into heparin and EDTA (T 0 ) or collected into heparin and incubated at 37°C for 10 min before addition of EDTA (T 10 ). The histograms represent the amount of C3 fragments detected on B220 + B cells following incubation of splenic lymphocytes with T 0 plasma (filled histogram) or T 10 plasma (open histogram), as measured by immunofluorescence flow cytometry. (B) Western blot analysis of C3 fragments in mouse plasma, as collected in A. C3 was immunoprecipitated from plasma samples with polyclonal anti–mouse C3 antibody conjugated Sepharose beads, and the Western blot was probed with biotin-labeled polyclonal anti–mouse C3 Ab and developed using streptavidin-HRP. Position of intact C3α and C3β chains and the C3dg fragments, that bind to CR1/2 on B cells (A), is indicated. (C) The level of C3 fragments in human blood collected directly into heparin and EDTA (T 0 ) or into heparin and incubated at 37°C for 30 min before addition of EDTA (T 30 ). The histograms represent the amount of C3 (solid line) or C3d (broken line) fragments detected on Raji cells after incubation with T 0 plasma (filled histogram) or T 30 plasma (open histograms), as measured by immunofluorescence flow cytometry. (D) The rate of formation of C3 fragments in blood from C57BL/6 mice collected into either heparin (□) or hirudin (▪) is depicted, as measured by the binding of C3 fragments to B220 + spleen cells using immunofluorescence flow cytometry, as shown in A. Error bars represent ± SD of means of triplicate assays.

Article Snippet: For human plasma samples, Raji cells were incubated with either a polyclonal goat anti–human C3-FITC antibody (Cappel; ICN Pharmaceuticals) or a polyclonal rabbit anti–human C3d-FITC antibody (Dako).

Techniques: Incubation, Ex Vivo, Clinical Proteomics, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation, Labeling, Binding Assay